Paper product with disinfecting properties

ABSTRACT

A paper product having an antimicrobial composition dispersed with the substrate is provided. In particular, a paper towel such as those found in public washrooms or institutions comprises an antimicrobial composition. The substrate can also be a facial tissue, kitchen towel, serviette or any type of personal hygeine product. The antimicrobial composition preferably comprises a phenol-derived antimicrobial agent. The paper products of the present invention appear dry to the touch.

FIELD OF THE INVENTION

The present invention relates to paper products containing anantimicrobial composition, particularly paper hand towels.

BACKGROUND OF THE INVENTION

Various types of microorganisms can have deleterious effects on humanhealth. Microorganisms are involved in soil and water contamination,food poisoning, skin infections, respiratory infections, bacteremia andviremia. It is well known that contaminated hands can spread manyinfections. For example, one can be exposed to the common cold fromshaking hands with someone who has just sneezed. Furthermore, diseasessuch as hepatitis can be spread to large numbers of people by aninfected individual handling food.

It is also well known that hand washing can remove many microbes and itis recommended that people wash their hands frequently to prevent thespread of disease. In certain environments, such as in food handling andin hospitals, there are strict hand-washing regulations. While washingwith soap may decrease the number of microorganisms on the hands, theprimary action of plain soap is the mechanical removal of viabletransient microorganisms. The effectiveness depends on the thoroughnessof the washing. In addition, the water itself may contain water-bornemicrobes or the hands can become re-contaminated by touching the tap ordoor handle.

Soap with water can physically remove a certain level of microbes, butantiseptic agents are necessary to kill or inhibit microorganisms andreduce the level still further. There is an increasing public awarenessof the health issues surrounding microbial contamination and thus thereis an increasing market demand for antimicrobial products. Some examplesof antimicrobial products include antibacterial hand soap, surgicaldisinfectants, household kitchen and bathroom cleansers, diaper wipes,deodorant, facial washcloths, hand wipes and other types of personalhygiene products.

U.S. Pat. No. 6,258,368 is directed to an antimicrobial wipe comprisingan absorbent sheet impregnated with an antimicrobial cleansingcomposition. The composition is pH adjusted in order to be less harsh onthe skin and to have a residual antimicrobial effect. The product isformatted as a wet wipe and it is necessary to maintain the moistnessfor efficacy.

U.S. Pat. No. 6,399,560 is directed to a biocidal composition and acloth incorporating the composition. The cleaning device is designed tobe effective against a wide range of bacteria without being undulytoxic. The biocidal composition requires the addition of a metalpyridinethione.

Canadian Patent No. 2,208,068 discloses a medicated tissue paper productthat carries a lotion for soothing irritated and sore nasal areas and amedicinal substance that is either dispersed directly in the lotion oris contained in microcapsules that are dispersed in the lotion. Thepaper product does not have antimicrobial properties.

U.S. Pat. No. 6,325,969 discloses a paper product impregnated with avolatile biocidal chemical to create a no-growth zone on the paper. Thetreated paper is not useful as an antimicrobial device.

Many of the commercially available disinfectants have high levels ofalcohol or harsh surfactants that tend to dry out and irritate the skin.In addition, wet wipes require special sealable packaging to preventdrying out which would result in a decrease in activity. Dryingtechniques also affect the efficacy of hand washing protocols. Forexample, it usually takes longer to dry hands with an air-dryer andpeople often do not take sufficient time to properly dry the hands. Theprimary problem with hand hygiene is not a lack of antimicrobialproducts, but rather a lack of compliance with required standards. Anumber of studies have looked at the influence of a variety of factorson hand washing behaviour.

Thus, there remains a real and unmet need for an effective andeasy-to-use sanitizing aid to help reduce microbial contamination.

SUMMARY OF THE INVENTION

It is an object of the present invention to provide a sanitizing productthat is safe and efficacious and does not require airtight packaging.

The present invention provides antimicrobial paper products that areessentially dry. An antimicrobial-containing composition is speciallyformulated so that it can be applied to a paper product. Theantimicrobial has activity in the dry state and its activity is enhancedwhen it is liberated from the paper product by contact with a fluid.

In one aspect of the invention a substantially dry-to-the-touch paperproduct is provided. The paper product comprises an antimicrobial activeagent dispersed on a support matrix. The antimicrobial agent may beprovided in a composition comprising a solubilizing agent. Theantimicrobial composition may also include a carrier to enhanceapplication to the matrix. The antimicrobial active agent is generally abacteriostatic or bacteriocidal agent, a virucide, a fungicide or adisinfectant and is preferably a phenol derivative. Exemplary phenolderivatives include 2-phenylphenol, sodium-2-phenylphenolate,hexachlorophene, chlorhexidine and diphenyl ethers.

In a preferred embodiment, the paper product is a paper hand towelcomprising a phenol-derived antimicrobial active agent on a substrate.

The antimicrobial active agent is typically dissolved in a solvent, suchas methanol, ethanol, ethylene glycol, isopropanol, polyglycol andpropylene glycol to provide an antimicrobial composition.

In a preferred embodiment, the antimicrobial composition comprises 5 to95% by weight of a phenol-derived antimicrobial agent and 5 to 95% byweight of a solvent, preferably 5-50% of each.

The antimocrobial composition may also include a carrier to facilitatetransfer to the paper. A preferred carrier is polyethylene glycol (PEG).

The paper product of the present invention may include an additionalcomponent, such as a surfactant, an alcohol, an ether, an emollient, ora fragrance.

In another aspect of the invention, the paper product is anantimicrobial hand towel that comprises a substrate and a phenol-derivedantimicrobial active agent disposed on the substrate.

The hand towel may be provided in a format for use in an institutionalenvironment or for use in a consumer environment. When the towel is usedto dry the hands, the antimicrobial is transferred onto the hands andcan reduce the microbial load on the hands.

The present invention also provides a process for sanitizing handscomprising washing the hands and drying them with the antimicrobial handtowel.

An antimicrobial composition for use on a paper towel is also provided.The composition comprises a phenol-derived antimicrobial agent and asolvent.

A phenol-derived antimicrobial composition can be applied to a paper webusing a variety of techniques. For example, spraying, flexographicprinting or roto gravure printing techniques can be used.

BRIEF DESCRIPTION OF THE DRAWINGS

The invention is described in more detail herein with reference to thedrawings, in which:

FIGS. 1 a and 1 b are schematics of flexographic printing systems;

FIG. 2 is a schematic illustrating a spray application method;

FIG. 3 is a schematic illustrating a roto gravure printing system;

FIG. 4 is a top view of an antimicrobial paper towel;

FIG. 5 is a cross-sectional view of a paper towel;

FIG. 6 is a graph indicating antimicrobial efficacy at 60 seconds; and

FIG. 7 is a graph indicating antimicrobial efficacy at 5 minutes.

DETAILED DESCRIPTION

The antimicrobial paper products of the present invention comprise apaper substrate and an antimicrobial active component.

As used herein, the terms “paper” and “substrate” are usedinterchangeably to refer to sheet material made from all natural filrousmaterials or a blend of natural and synthetic and/or semi-syntheticfibrous materials. Examples of sheet material include institutionalpaper hand towels, napkins, serviettes, facial tissue paper, toiletpaper, household paper towels and the like.

The paper substrate of the present invention can be prepared usingconventional methods. For example, a non-woven substrate comprisingfilamentous fibers having a web structure can be used. A substratehaving a random distribution can also be used. In general, any standardsubstrate can be used as long as it has an appropriate tensile strengthand absorbency. Useful substrates for the practice of the inventiontypically have a basis weight in the range of about 8 to about 30 poundsper ream and total water absorption capacity of approximately 90 to 400grams per square meter.

The term “antimicrobial” refers to a biocidal or biostatic compoundwhich controls the viability and/or proliferation of microorganisms. Theantimicrobial composition is active against bacteria, mould, fungiand/or yeast. The antimicrobial composition can also be formulated tohave a virucidal effect. The antimicrobial agent may be referred to asan anti-bacterial, an anti-mycotic, a virucide, a fungicide, asanitizer, a disinfectant, a biocide or the like. The antimicrobialagent may be biologically or chemically derived. The antimicrobial agentis preferably provided in an antimicrobial composition which can beapplied to the substrate. The term “sanitize” is used broadly herein torefer to a reduction in microbes and not necessarily completesterilization.

The antimicrobial composition of the present invention comprises asolvent and an antimicrobial active agent. The composition mayoptionally include a surfactant and/or an additional carrier.

The antimicrobial composition comprises from about 5% to about 95%,preferably 15% to 40% by weight of the antimicrobial active agent.Antimicrobials that are useful in the present invention include phenoland phenol derivatives. Some exemplary antimicrobial agents are2-phenylphenol, sodium-2-phenylphenolate, hexachlorophene,chlorhexidine, diphenyl ethers, etc. A preferred antimicrobial is2-phenylphenol. The hydrated sodium salt, sodium 2-phenylphenolate andother types of phenol derivatives may also be used. Some exemplaryderivatives are available from Bayer under the trademark Preventol™. Itis clearly apparent that other antimicrobials having the same propertiesin terms of biocidal activity and solubility could also be used. Thephenol derivatives useful in the present invention have low toxicity andgood biodegradability.

The antimicrobial active component is dispersed in a solvent medium orsystem to solubilize the active ingredients. Preferred solvents arealcohol or ether based and the solvent system is preferably compatiblewith water. Some examples of solvents include, but are not limited to,methanol, ethanol, ethylene glycol, isopropanol, polyglycol, propyleneglycol and mixtures thereof. In a preferred embodiment, a glycol solventsystem is used. One preferred solvent is propylene glycol, but it isclearly apparent that any other solvent, which is compatible withapplication to paper (i.e. not highly volatile, low % water) and whichis safe for use by humans, can be used.

A carrier may be added to improve the consistency of the composition.The composition may include polyethylene glycol (PEG) as an extender orco-solvent. The PEG provides added viscosity and is water compatible andfacilitates the transfer of the composition to the paper.

The selected antimicrobial is formulated into a composition thatprovides for a substantially “dry” paper product. In other words, thepaper feels more like a dry paper towel than a wet wipe.

The antimicrobial compositions of the present invention are formulatedto be compatible with dry paper. It is well known that chemicalcompositions can be added at the wet end of the paper making process.However, when this is done, expensive additives may be may lost in thewhite water drained from the paper and treatment of the waste effluentmay be required prior to environmental release. On the other hand, thewater in aqueous compositions applied at the dry end of the process canhave detrimental effects on the strength and flexibility of the paperproduct. The compositions of the present application are formulated sothat they can be added at the dry end of the process without anysignificant detrimental effects. This extends the application of thecomposition to grades of paper that would not normally be conducive tothe addition of a water-based composition at the dry end. Addition ofthe composition at the dry end also minimizes waste and provides for amore cost-effective use of the antimicrobial. The resultantantimicrobial paper products feel essentially dry to the touch. Theactive ingredient of the antimicrobial composition is stable in the drystate and thus the “dry” product has a long shelf life. Theantimicrobial is released primarily when the product is wetted.

The antimicrobial composition can be applied to the paper product usingvarious standard techniques such as flexographic printing, spraying orroto gravure printing.

FIGS. 1 a and 1 b generally illustrate the process of applying theantimicrobial composition to a substrate by a flexographic press. InFIG. 1 a, a plain web of paper 10 is fed between a backing roll 12 andplate roll 14 which counter rotate. The antimicrobial composition 17 isplaced in a fountain or ink pan 16 and is picked up by the pick up roll18 and then transferred to the anilox roll or cylinder 20. The aniloxroll has engravures or etchings 19 so as to transfer the composition tothe plate roll 14 to coat the web 10. The printed web 22 is then rolled,cut and/or folded according to the desired format.

A variation of the flexographic press system is illustrated in FIG. 1 b.The system comprises a closed cavity system with doctor blades 24 tocontrol the amount of antimicrobial applied to the substrate. AlthoughFIG. 1 illustrates the disinfecting composition being applied to oneside of the web, it is clearly apparent that both sides of the web maybe coated.

Variations in flexographic printing techniques are contemplated. Forexample a flexographic press with an “all over” coating roller may beused to apply the composition using standard techniques.

FIG. 2 illustrates how the antimicrobial composition can be appliedusing a spray technique. In the illustration, a roll 31 is comprised ofa single ply substrate 32, but it clearly apparent that a multi-plysubstrate could also be used. Idler rollers 33 help to keep tension onthe substrate web. The antimicrobial solution 35 is mixed with air 36 ina spray nozzle 34 and then applied as an aerated solution 37 to thesubstrate. The antimicrobial-coated web of paper 39 leaves the sprayingzone and proceeds to the next operation (e.g. drying unit, folder,etc.). If the antimicrobial composition is to be sprayed, appropriatecontainment measures must be taken to address health and safety issues.For example, a closed system could be used.

FIG. 3 illustrates schematically a roto gravure press having a backingroll 40, such as a rubber impression roll. The press also includes agravure roll 42 which contacts the antimicrobial composition 44. Thisinteraction coats the underside 46 of the web.

In addition to the techniques illustrated in FIGS. 1, 2 and 3, the paperproduct of the present invention can be prepared using other standardpaper production techniques.

FIG. 4 illustrates schematically a paper towel 50 on which theantimicrobial composition 52 has been flexographically printed in ahoneycomb pattern. Although the composition 52 is initially applied tothe honeycomb walls 54, there may be a capillary effect which isdependent on the type of substrate used. This effect allows theantimicrobial composition to disperse into the cores 56. It is clearlyapparent that various other printing patterns can be used and that theamount of dispersion depends on both the printing pattern and the typeof paper substrate used.

FIG. 5 is a schematic cross-section through a paper towel 60 that hasbeen printed on both sides 62, 64 with an antimicrobial composition. Theprinting may or may not result in compression of the paper product.

In a preferred method of the present invention, a stock solution isprepared which has an antimicrobial active concentration of 50% byweight. The stock solution can be stored and used in the preparation ofdifferent formulations of antibacterial compositions.

Table 1 illustrates a few examples of various formulations ofantimicrobial compositions that were prepared and tested in aflexographic printing system.

Table 2 illustrates some examples of formulations of antimicrobialcompositions that were prepared and tested using a roto gravure printingsystem.

The following abbreviations are used in the tables:

-   -   OX=OPP=ortho-phenylphenol (e.g. Preventol O Extra from Bayer AG)    -   ONX=SOPP=sodium ortho-phenylphenolate (e.g. Preventol ON Extra        from Bayer AG)    -   PG=propylene glycol (e.g. USP grade from Ashland Chemical)    -   PEG_(abc)=polyethylene glycol where a, b, c represent integers        for the average gram molecular weight of the distribution (e.g.        Carbowax from Union Carbide)    -   PVOH=polyvinyl alcohol (e.g. Vinol 205 crystals by Air Products        and Chemicals Inc.)    -   PVOH 8%=Vinol 205 used as 8% by weight of solution in process        water    -   Cetylol=cetyl alcohol powder (e.g. Kalcol 6098 by KAO        Corporation)    -   APS=stearyl ether with propylene oxide (e.g. Varionic APS from        Goldschmidt Chemical Corp.)    -   Surf 365=ethoxylated Castor Oil (e.g. Surfactol 365 from CasChem        Inc.)    -   nPrOH=normal propanol or propyl alcohol

In both tables, the values for the various components are presented as apercent by weight ratio. In a preferred embodiment, the antimicrobialcomposition comprises 30 to 45% by weight of a phenol-derivedantimicrobial agent, 30 to 45% by weight of a solvent, and 10 to 40% byweight of a carrier.

More preferably, the composition comprises 35 to 42% by weight of2-phenylphenol; 35 to 42% by weight of propylene glycol; and 16 to 30%by weight of a polyethylene glycol.

Referring to the rows labeled “% ADD-ON” on each table, it can be seenthat the various antimicrobial compositions were effectively loaded ontothe substrate. The extent of loading (% ADD-ON) was calculated using thefollowing formula:

$\frac{{Net}\mspace{14mu}{Weight}\mspace{14mu}{of}\mspace{14mu}{Liquid}\mspace{14mu}{Mixture}\mspace{14mu}{Coating}}{{Net}\mspace{14mu}{weight}{\mspace{11mu}\;}{of}\mspace{14mu}{Plain}\mspace{14mu}{Paper}\mspace{14mu}{Used}} \times 100\%$

FORMULAE FOR ANTIBACTERIAL LIQUIDS USED FOR COATING PAPER BY GRAVUREPRESS TRIAL: CT-17 CT-18 CT-19 CT-20 CT-21 CT-22 Ingredient (Proportionas Percentage by Weight of Mixture) OPP = OX 32.26% Same as ø  39.86%29.66% 25.06% Formula CT-17 SOPP = ø ø ø ø ø ONX PG 32.26% ø  39.86%29.66% 40.56% PEG 400 32.09% ø ø ø ø PEG 600 ø  98.0%  19.88% 39.86%33.68% PEG 3350  0.16% ø ø ø ø PEG 8000 ø  2.0%  0.41%  0.81%  0.69%PVOH 8%  2.67% ø ø ø ø Cetylol  0.56% ø ø ø ø TOTALS: 100.0% 100.0%100.01% 99.99% 99.99% ADD-ON 6.57 3.54 4.22 11.38 21.89 2.58 (%) SPEED500 1500 500 500 500 1500 (FPM)

FORMULAE FOR ANTIBACTERIAL LIQUIDS USED FOR COATING PAPER BY FLEXOGRAPHYTRIAL: FX-1 FX-2 FX-3 FX-4 FX-5 FX-6 FX-7 FX-8 FX-23 FX-24 FX-25 FX-26MS-1 OPP = OX ø 18.64 14.79 13.31 37.93 23.50 11.74 9.40 ø ø 9.67 7.4419.49 SOPP = ø ø ø ø ø ø ø ø 15.09 7.51 7.53 10.84 ø ONX PG ø 18.6414.79 13.32 37.93 23.50 11.74 9.40 ø ø 40.07 14.86 49.62 PEG 200 100.062.73 49.79 44.82 24.14 14.96 52.48 42.02 ø 50.24 35.19 56.02 PEG 400 =30.89 APS ø ø 20.63 28.55 ø ø ø ø ø ø ø ø ø SURF 365 ø ø ø ø ø 38.0424.04 19.25 ø ø ø ø ø NPrOH ø ø ø ø ø ø ø 19.93 ø ø ø ø ø DIW ø ø ø ø øø ø ø 84.91 42.25 7.54 10.84 ø TOTALS 100 100.01 100.00 100.00 100.00100.00 100.00 100.00 100.00 100.00 100.00 100.00 100.00 ADD-ON 3.90 3.363.02 3.27 4.71 3.25 2.11 3.27 4.84 5.46 4.50 3.33 — (%) SPEED 186 600604 600 600 500 600 600 250 400 425 460 — (FPM) VISCOSITY — 20.25 24.527.2 27.6 36.0 19.1 11.6 — — 14.2 14.5 —

Referring to Table I, it can be seen that the FX-5 run in particular hada good deposition of the ortho-phenyphenol (OPP).

Using a gravure press as indicated in Table 2, the CT-20 and CT-21 runsdemonstrated a high degree of loading of the antimicrobial active agentonto the substrate.

To determine the actual content of actives (% actives) applied onto thepaper substrate, the following calculation can be done:

$\frac{\%\mspace{14mu}{Active}\mspace{14mu}{Component}\; \times \%\mspace{14mu}{Add}\text{-}{On}}{100\%}$

The antimicrobial efficacy of the finished product can be assessed inseveral ways.

For example, a small square can be cut from the antimicrobial towel andplaced in a bag with sterile water. An aliquot of a bacterial culture isadded and at several time intervals, a sample is removed and a platecount is performed. The bacterial counts in the paper towel treatedsamples are compared to a control sample that was cultured in theabsence of treated paper towel.

In one aspect of the invention a paper product that is biocidal to avariety of microorganisms is provided. Samples of paper fromflexographic (FX) and rotogravure (CT) runs were taken and tested fortheir biocidal effect against Salmonella choleraesuis, Escherichia coliand Staphylococcus aureus as described more fully in Example 3 below.Culture samples were taken after exposure to the paper for 15 sec, 1 minand 5 min. Table 3 below indicates the log₁₀ reduction factor for aSalmonella choleraesuis culture.

TABLE 3 Time (minutes) PRODUCT 0.2 1 5 FX-2 0.17 0.22 0.90 FX-5 0.510.99 4.5 CT-20 1.35 3.19 3.74 CT-21 1.25 1.57 2.36

In another embodiment, an anti-E. coli paper product is provided. Theefficacy against E. coli was assessed using the same protocol asdescribed above and in Example 3. These results are shown in Table 4below.

TABLE 4 Time (minutes) PRODUCT 0.2 1 5 FX-2 0.01 0.03 0.72 FX-5 0.500.86 0.97 CT-21 0.61 0.86 0.96In yet another embodiment, a paper product effective againstStaphylococcus aureus is provided. The efficacy results are shown inTable 5 below.

TABLE 5 Time (minutes) PRODUCT 0.2 1 5 FX-2 0.00 0.01 0.40 FX-5 0.072.59 2.81 CT-21 0.00 0.01 2.11

The results indicate that all of the samples of antimicrobial paper hadantimicrobial activity against Salmonella choleraesus, Escherichia coliand Staphylococcus aureus and that the effect increased with theexposure time. The paper products of the present invention have alsobeen shown to be effective against the organism Serratia marcescens asdescribed in Example 4 below. The results indicate that the paperproducts are effective against a variety of both gram positive and gramnegative organisms.

It should be noted that the initial innoculum count for each of theorganisms was very high, approximately 85×10⁵. Thus, this is a veryonerous test for the paper and even a small log₁₀ reduction factorrepresents a significant biocidal and/or biostatic effect. It is clearlyapparent that a much greater log₁₀ reduction factor can be achievedusing a lower bacterial load, in the range of that which would beexpected on hands that had just been washed.

It has also been shown that the product is stable. In a similar assayusing Salmonella choleraesius, the efficacy of an FX-5 sample taken twoweeks after the run was compared to the efficacy of a sample from thesame run after storage for approximately eight months. The two weeksample showed a Log₁₀ decrease in CFU/ml of 0.51, 0.99 and 4.5 at 15sec., 60 sec. and 300 sec. respectively. The eight month sample showed aLog₁₀ decrease in CFU/ml of 0.61, 1.73 and 3.43 at 15, 60 and 300seconds, respectively. It is clearly apparent that long-term storage didnot significantly affect the antimicrobial activity of the product.

A comparison of the results obtained for FX-2 and FX-5 suggests that theantimicrobial efficacy of the sample correlates with the % ADD-ON of theactive agent.

FIGS. 6 and 7 further illustrate the effect of the % Add-On on theability to kill Salmonella in sixty seconds and five minutes,respectively.

The antimicrobial activity of the product can also be assessed bypreparing a bacterial culture on a growth medium and placing a sample ofthe paper product on/in the medium. The biocidal and/or biostaticeffects can be assessed visually by observing a ring of non-growtharound the paper.

Another way to test the efficacy of the paper product is to incubate abacterial culture in the presence of a sample of antimicrobial paper ora sample of control paper. After a pre-determined incubation time, analiquot is removed and a bacterial count is done.

Alternatively the paper product can be rinsed and the run-off tested foractivity. Standard microbiological assays for biocidal/biostaticactivity are well know to those skilled in the art and can be used todetermine the antimicrobial activity of the product.

The anti-viral and anti-fungal properties can be assessed in similarways. For example, viral infectivity of cells in the presence of thepaper product can be determined. Generally, the antimicrobial propertiesof the treated paper product can be assessed using a variety of standardmicrobiological assays.

The in vivo efficacy of the products can also be determined. Forexample, the bacterial load on an individual's hands can be assessedbefore and after drying the hands with a paper towel treated accordingto the present invention. One hand is dried with an antimicrobial papertowel and the other hand is dried with a regular paper towel. The handsare then rinsed with sterile water and a bacterial count is done.

The residual effect on re-contamination can also be assessed. Forexample, at a time after drying, treated and untreated hands can berinsed with sterile water and samples of the rinsate can be plated todetermine the bacterial count. Alternatively, the hands can be directlycontacted with a microorganism growth medium.

The in vivo efficacy can also be demonstrated using different types ofassays well known to those skilled in the art. In one such assay, asurface is coated with a bacterial solution. The surface is then driedwith an antimicrobial paper product of the present invention. Thesurface is then rinsed and the number of residual bacteria in therinsate is determined. The effect of the paper product can be assessedby determining the decrease in bacteria.

The results discussed herein demonstrate that antimicrobial paper towelsprepared according to the present invention are effective for infectioncontrol. The antimicrobial paper products of the present invention arehighly effective against a wide variety of microorganisms.

The antimicrobial paper products of the present invention are useful ina variety of ways. The antimicrobial paper product is preferably a paperhand towel.

In practice, a person washes their hands and then uses the antimicrobialpaper towel to dry them. The friction generated by the engagement of thehands and the towel transfers the antimicrobial agent to the person'shands. The water on the person's hands may also act to liberate andactivate the antimicrobial ingredient from the dry paper towel. Thehands become sanitized as they are dried. In addition to thepre-existing microorganisms on the hands, undesirable microbes in thetap water are also affected. Antimicrobial paper hand towels are ofparticular value in environments such as food handling stations andhospital settings. Because the antimicrobial is not rinsed off the handsafter drying, there is an ongoing residual antimicrobial effect.Antimicrobial paper towels may be provided as a rolled product, a foldedsheet product or a pre-packaged single use product.

In another aspect of the invention, the antimicrobial paper product is afacial tissue. When a person uses the tissue to, for example, blow theirnose, the microbial load on the tissue is decreased before it isdisposed of, thereby reducing the spread of germs. In addition, theantimicrobial ingredient is active in the dry state and may be passedonto the hands, further reducing the spread of disease.

The antimicrobial paper product may comprise a kitchen towel. The towelcan be dampened and used to clean surfaces. Alternatively, a dry towelcan be used to dry surfaces that were wet with water and provide aresidual antimicrobial effect.

The release of the antimicrobial from the antimicrobial paper product inthe presence of an exogenous fluid is also a useful feature forantimicrobial toilet paper and other personal hygiene products.

The paper products of the present invention may include, in addition tothe antimicrobial, other additives. For example, a skin protectant ormoisturizer can be included. Additives such as stabilizers, chemicaladditives, astringents, binders, fragrances, emollients and a widevariety of other compounds, as will be apparent to those skilled in theart, can also be included. The paper product may comprise more than onetype of antimicrobial. For example, a paper towel could be impregnatedwith an anti-bacterial and an anti-fungal. Various combinations ofadditives are contemplated.

The paper products of the present invention provide significantadvantages over current antimicrobial wipes. The products areessentially dry and therefore easy to store. The antimicrobial isreleased from the paper fibres in the presence of water. The productsare stable since the antimicrobial active agent has a long shelf life,especially in the dry form. The high degree of specialty packaging thatis necessary for wet wipes is not required for the present inventionsince there is no need, or even desire, to keep the product moist. Inaddition, the products of the present invention do not contain highamounts of alcohol or other agents which can irritate the skin.

The above disclosure generally describes the present invention. A morecomplete understanding can be obtained by reference to the followingspecific Examples. These Examples are described solely for purposes ofillustration and are not intended to limit the scope of the invention.Changes in form and substitution of equivalents are contemplated ascircumstances may suggest or render expedient. Methods of paper making,chemistry and microbiology referred to but not explicitly described inthis disclosure and examples are well known to those skilled in the art.

EXAMPLES

The examples are provided for purposes of illustration. Althoughspecific terms have been employed herein, such terms are intended in adescriptive sense and not for purposes of limitation.

Example 1 Preparation of a Stock Antimicrobial Composition

To prepare a 2-phenylphenol stock mix, Preventol O Extra™ was purchasedfrom Bayer. A stock of propylene glycol was heated slowly over one hourto a temperature of approximately 60° C. Preventol, in the form ofcrystalline flakes, was added slowly in four equal aliquots for a finalconcentration of 50% by weight of the stock solution. The solution wasmixed for 15 minutes and the heating was stopped. After approximately 15minutes more, the stock solution was transferred to a drum. The stocksolution can be stored and used in the preparation of differentformulations of antibacterial compositions.

Example 2 Preparation of an Antimicrobial Paper Product

A stock solution comprising equal parts by weight of 2-phenylphenol andpolyglycol was prepared. Approximately 25% by weight of PEG 200 wasadded. The resulting composition had a viscosity of approximately 200 cPor mPs. The composition was applied to a paper towel web using aflexographic printing technique with the web moving at a speed ofapproximately 600 FPM.

Example 3 Antimicrobial Efficacy of Flexographic Printed Towels

Two examples of antimicrobial paper towel were tested for their efficacyagainst Salmonella choleraesuis (ATCC 14028), Escherichia coli (ATCC8739) and Staphylococcus aureus (ATCC 6538). Samples from twoflexographic runs, termed FX-2 and FX-5, were cut into 10 cm×10 cmsquares (100 cm²). The two samples, FX-2 and FX-5 were printed withdifferent antimicrobial compositions as defined in Table 1. The piecesof paper were placed in a sterile bag and 1 ml. of sterile water wasadded to each. A 0.5 ml. aliquot of a 24-hour culture of one of the testorganisms was then added. After contact times of 15 sec., 1 min., and 5min., 10 mls of Letheen Broth was added per each piece of paper and bagwas agitated. A sample was then taken and a plate count assay was set upin duplicate for each piece. The plates were incubated at 30-35° C. for48 hours. The initial inoculum count for each of the organisms is asfollows:

S. choleraesuis: 88 × 10⁵ E. coli: 79 × 10⁵ S. aureus 90 × 10⁵

Media controls comprising TSA, Letheen Broth or normal saline did notshow any growth.

The efficacy results are shown in the following table.

TABLE 6 Organism FX-2 FX-5 S. choleraesuis 15 sec. 59 × 10⁵ 27 × 10⁵  1min. 53 × 10⁵ 91 × 10⁴  5 min. 11 × 10⁵ 28 × 10¹ E. coli 15 sec. 78 ×10⁵ 25 × 10⁵  1 min. 79 × 10⁵ 11 × 10⁵  5 min. 15 × 10⁵ 85 × 10⁴ S.aureus 15 sec. 89 × 10⁵ 76 × 10⁵  1 min. 88 × 10⁵ 23 × 10³  5 min. 36 ×10⁵ 14 × 10³

These results indicate that the antimicrobial paper towels are effectiveagainst both gram-positive and gram-negative bacteria.

Example 4 Efficacy Against Serratia marcescens

One flexograph sample and two rotogravure samples were tested forantimicrobial efficacy against the microorganism, Serratia marcescens(ATCC14756). The compositions used for the printing are outlined inTable 2 above. Samples FX-5, CT21 and CT22 were cut in 100 cm² squaresand the squares were placed in sterile bags. One milliliter of eithersterile reagent-grade (deionized and purified by Millipore unit) wateror sterile tap water (drinking water from Orangeville, Ontario) wasadded to each sample. An aliquot of 0.5 ml of the test organism was thenadded. After 15 sec, 1 min and 5 min, 10 ml of Letheen Broth was addedto each bag and agitated. Plate counts were then performed in duplicateas described above.

The initial inoculum count was 72×10⁷. The results of the assay areshown in Table 7 as follows.

TABLE 7 Diluent FX-5 CT-22 CT-21 Millipore water 15 sec. 15 × 10⁷ 65 ×10⁷ 24 × 10⁷  1 min. 25 × 10⁶ 62 × 10⁷ 20 × 10⁷  5 min. 21 × 10⁶ 23 ×10⁷ 11 × 10⁷ City water 15 sec. 12 × 10⁷ 31 × 10⁶ 26 × 10⁷  1 min. 46 ×10⁶ 27 × 10⁷ 21 × 10⁷  5 min. 30 × 10⁶ 15 × 10⁷ 14 × 10⁷

These results indicate that different samples of paper towel areeffective anti-bacterial agents.

Example 5 In Vivo Assay for Antimicrobial Activity

To test the in vivo efficacy of a hand towel according to the presentinvention, an aliquot of a bacterial culture is applied to anindividual's hands. The hands are washed and then dried using a regularpaper towel or an antimicrobial paper towel of the present invention.The hands are then rinsed with sterile water and a bacterial count isperformed on the rinse.

Although preferred embodiments of the invention have been describedherein in detail, it will be understood by those skilled in the art thatvariations made be made thereto without departing from the spirit of theinvention or the scope of the appended claims.

I claim:
 1. A dry paper towel comprising a substrate and a singleantimicrobial composition disposed on said substrate, wherein saidcomposition is surfactant-free and said composition comprises ahydrophilic solvent in which is dispersed only a single antimicrobialactive agent, wherein said antimicrobial active agent is 2-phenylphenoland wherein the paper towel exhibits a log 10 decrease in CFU/ml of atleast about 0.1 for an exposure of 15 minutes.
 2. The paper towelaccording to claim 1, wherein the hydrophilic solvent is selected fromthe group consisting of methanol, ethanol, ethylene glycol, isopropanol,polyglycol, propylene glycol and mixtures thereof.
 3. The paper towelaccording to claim 1, wherein the antimicrobial composition comprisesfrom about 5% to about 95% by weight of the antimicrobial active agent.4. The paper towel according to claim 3, wherein the antimicrobialcomposition comprises from about 5% to about 50% by weight of theantimicrobial active agent.
 5. The paper towel according to claim 2,wherein the solvent comprises propylene glycol.
 6. The paper towelaccording to claim 1, wherein the antimicrobial composition furthercomprises a carrier.
 7. The paper towel according to claim 6 wherein thecarrier comprises polyethylene glycol and the antimicrobial compositioncomprises about 16% to about 30% of the carrier by weight.
 8. The papertowel according to claim 7, wherein the carrier is selected from thegroup consisting of PEG200, PEG400, PEG600, PEG3350, PEG8000 andmixtures thereof.
 9. The paper towel according to claim 1, wherein theantimicrobial composition further comprises an additional componentselected from the group comprising consisting of an alcohol, anemollient, a fragrance and combinations thereof.
 10. A rolled productcomprising the paper towel according to claim
 1. 11. A folded sheetproduct comprising the paper towel according to claim
 1. 12. Apre-packaged, single use product comprising the paper towel according toclaim
 1. 13. A paper product that exhibits a log 10 decrease in CFU/mlof at least about 0.1 for an exposure of 15 minutes, said paper productcomprising a substrate selected from the group consisting of a paperhand towel, a napkin, facial tissue, toilet paper and household papertowels and a single antimicrobial composition wherein said antimicrobialcomposition is surfactant-free and comprises 30 to 45% by weight of ahydrophilic anhydrous solvent in which is dispersed 30 to 45% by weightof only a single antimicrobial agent, wherein said agent is aphenol-based antimicrobial agent, and 10 to 40% by weight of a carrier.14. The paper product according to claim 13, wherein the antimicrobialcomposition comprises: i) 35 to 42% by weight of 2-phenylphenol; ii) 35to 42% by weight of propylene glycol; and iii) 16 to 30% by weight of apolyethylene glycol.
 15. A dry paper towel comprising a substrate and asingle antimicrobial composition disposed on said substrate, whereinsaid composition is biodegradable and surfactant-free, said compositioncomprising a hydrophilic solvent in which is dispersed a phenol-basedantimicrobial active agent, wherein the paper towel exhibits a log 10decrease in CFU/ml of at least about 0.1 for an exposure of 15 minutes.16. The paper product according to claim 13, wherein the phenol-basedantimicrobial agent is selected from the group consisting of2-phenylphenol, sodium-2-phenylphenolate, hexachlorophene, chlorhexidineor diphenyl ethers.
 17. The paper product according to claim 15, whereinthe phenol-based antimicrobial agent is selected from the groupconsisting of 2-phenylphenol, sodium-2-phenylphenolate, hexachlorophene,chlorhexidine or diphenyl ethers.
 18. A method of preparing anantimicrobial paper product as defined in claim 13, said methodcomprising applying to the paper substrate the surfactant-freeantimicrobial composition.
 19. The method of claim 18, wherein saidcomposition is applied using a process selected from the groupconsisting of spraying, flexographic printing and roto gravure printing.20. The method of claim 18, wherein the paper product is a paper towel.